Composite

Part:BBa_K3772011:Design

Designed by: Xiaoyu Wu   Group: iGEM21_SCUT-China   (2021-10-01)


ALD4p-VS-SAG1t expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1805
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is codon optimized by Sangon Biotech. (https://www.sangon.com/services_gene_mmzyhhjysjfw.html) The parts are connected together by enzyme digestion



Source

ALD4p and SAG1t are cloned from the promoter of the coding sequence for aldehyde dehydrogenase from the genome of saccharomyces cereviciae BJ5464-NpgA； VS is synthesized by Sangon Biotech according to GenBank:JX040471.1；

References